What type of ELISA is used for pregnancy test?
Principle of the test: The Visual hCG ELISA Test is a sandwich enzyme immunoassay (8-9) for the determination of human chorionic gonadotropin in urine or serum. The method employs two monoclonal antibodies to selectively identify hCG in urine/serum with a high degree of sensitivity.
Which disease is diagnosed through Elisa test?
The assay used most widely to detect or diagnose virus infection, especially infection of blood borne viruses e.g. HBV, HCV, HIV and HTLV, is the enzyme linked immunosorbent assay (ELISA), whose sensitivity and practicability have rendered it the most common primary screening assay.
How does pregnancy test show positive?
Pregnancy tests check your pee or blood for a hormone called human chorionic gonadotropin (hCG). Your body makes this hormone after a fertilized egg attaches to the wall of your uterus. This usually happens about 6 days after fertilization. Levels of hCG rise quickly, doubling every 2 to 3 days.
What is DNA biosensor?
DNA biosensors, based on nucleic acid recognition methods, are being developed towards the assay of rapid, simple and economical testing of genetic and infectious diseases. … Unlike enzyme or antibodies, nucleic acid recognition layers can be readily synthesized and regenerated for multiple uses.
How do pregnancy tests work?
Pregnancy tests work by checking your urine (pee) for a hormone called human chorionic gonadotropin (HCG). Your body only makes this hormone if you’re pregnant. HCG is released when a fertilized egg attaches to the lining of your uterus — when pregnancy begins.
When does hCG test positive in urine?
hCG is a hormone produced by your placenta when you are pregnant. It appears shortly after the embryo attaches to the wall of the uterus. If you are pregnant, this hormone increases very rapidly. If you have a 28 day menstrual cycle, you can detect hCG in your urine 12-15 days after ovulation.
What happens if you pee too much on a pregnancy test?
The hook effect happens when you have too much hCG in your blood or urine. How is this possible? Well, the high levels of hCG overwhelm the pregnancy test and it doesn’t bond with them correctly or at all. Rather than two lines saying positive, you get one line that incorrectly says negative.